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Port 25 blocking When an ISPs block its port 25 does not total block it, what it does is to check the sender IP  or email domain listed on their "blacklist" database and block your mail server from sending mails to it.
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About Port 25 blocking

Using your PCs as a mail server, you won't be able to send-out emails to recipients whose domain reside in ISPs that have blocked their port 25 

ISPs (Internet Service Providers) are blocking Port 25 because all mails sent via the Internet is routed through it. Because it is the standard communication SMTP channel used for communication between a mail client (ie: Eudora, Netscape Communicator, Outlook) and a mail server.

When an ISPs block its port 25 it does not literally (total) block it, what it does is to check the sender IP or email domain that is listed on their "blacklist" database and block your mail server from sending mails to it.

For example: If you are sending a lot of email messages to the same email domain like; hotmail.com, aol.com, or yahoo.com their SMTP mail servers may block your email domain or IP after a certain number of email messages--because they may have set their mail servers to block anyone that have sent more than 100 messages within a short period of time.

In other words, they can limit the volume of emails from any mail server by time and/or quantity as well as permanently barred (blacklist) an email domain or IP so that in future all all email from the IP or domain will be automatically deleted without notifying the sender.

Please note that the PORT 25 that your program uses to send email messages to recipient mailboxes and the port the program uses to establish connections with your email program is not the same thing.  See: Port Numbers

 

 
 
 
 

You can use any port, including PORT 25 to establish a connection between your email program and your (desktop PCs) local SMTP mail server program...
but

When your local SMTP
mail server program actually deliver your email messages to the recipients mailboxes, it always use PORT 25 to transmit data to the remote mail server - because it is the default standard SMTP port. Therefore, if your ISPs have block it, your mails won't get through.

Port 25 blocking a method used by ISPs like: Juno, Netzero, FunCow, AT&T, MSN, etc. is one of the ways, to prevent spammers from using random ISPs to send unsolicited emails.
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